Mackie and Norwood start at BoltonSteve Clarke has made two changes to the side that lost at Forest last weekend ahead of tonight's clash at Bolton's Macron Stadium.
Jamie Mackie, who was ineligible to play against his parent side on Saturday afternoon, replaces Simon Cox in the starting eleven with Cox taking his place on the bench.
And Oliver Norwood is drafted into the starting line-up as Danny Williams begins the game as a substitute.
Jake Cooper returns to the squad to take a place amongst the substitutes, but Jure Travner and Danny Guthrie miss out.
Bolton start with former Royals Matt Mills and Adam Le Fondre, while Emile Heskey is not included in the squad. Eidur Gudjohnsen is a substitute for Neil Lennon's men.
Bolton: Amos, McNaughton, Wheater, Mills (c), Ream, Danns, Bannan, Feeney, Janko, Clough, Le Fondre.
Substitutes: Fitzsimons, Moxey, Dervite, Gudjohnsen, Vela, Eaves, Walker.
Reading: Federici, Gunter, Obita, Pearce (c), Hector, Chalobah, Norwood, Mackie, Robson-Kanu, McCleary, Pogrebnyak.
Substitutes: Andersen, Kelly, Cooper, Williams, Blackman, Cox, Yakubu.