17-year-old defender Tom Holmes starts tonight, making his professional debut at the back for Jaap Stam's side to become the sixth Academy graduate to break into the first team this season.
Anssi Jaakkola also comes into the eleven to start between the sticks, while Dave Edwards, George Evans, Sone Aluko and Mo Barrow are also included in the line-up as manager Jaap Stam makes six changes to the side that lost at home to Sheffield United here a week ago.
John Swift returns to the squad to take a place on the bench, while Garath McCleary, Omar Richards and Leandro Bacuna join Yann Kermorgant, Stephen Quinn, Jordan Obita, Paul McShane and Axel Andresson on the injury list.
Joseph Mendes and Callum Harriott returned from long-term injuries to play in an Under-23s win over West Brom on Monday night but are not fit enough for first team contention yet.
As for Bolton, former Royals Jem Karacan, Andrew Taylor and Adam Le Fondre are both in the squad, with the latter two starting at Madejski Stadium on Tuesday night.
Reading: Jaakkola, Gunter (c), Moore, Holmes, Edwards, Clement, Kelly, Evans, Aluko, Barrow, Martin.
Subs: Mannone, Blackett, Ilori, Van den Berg, Swift, Bodvarsson, Smith.
Bolton Wanderers: Alnwick, Taylor, Beevers, Kirchhoff, Flanagan, Pratley, Henry, Burke, Morais, Buckley, Le Fondre.
Subs: Howard, Dervite, Karacan, Noone, Robinson, Wilbraham, Clough.